三氟乙酸
肽
选择性
色谱法
甲酸
质谱法
化学
电喷雾
高效液相色谱法
分析化学(期刊)
有机化学
生物化学
催化作用
作者
Asish B. Chakraborty,Scott B. Berger
出处
期刊:Journal of biomolecular techniques
[Association of Biomolecular Resource Facilities]
日期:2005-12-01
卷期号:16 (4): 327-335
被引量:6
摘要
The balance between chromatographic performance and mass spectrometric response has been evaluated using an automated series of experiments where separations are produced by the real-time automated blending of water with organic and acidic modifiers. In this work, the concentration effects of two acidic modifiers (formic acid and trifluoroacetic acid) were studied on the separation selectivity, ultraviolet, and mass spectrometry detector response, using a complex peptide mixture. Peptide retention selectivity differences were apparent between the two modifiers, and under the conditions studied, trifluoroacetic acid produced slightly narrower (more concentrated) peaks, but significantly higher electrospray mass spectrometry suppression. Trifluoroacetic acid suppression of electrospray signal and influence on peptide retention and selectivity was dominant when mixtures of the two modifiers were analyzed. Our experimental results indicate that in analyses where the analyzed components are roughly equimolar (e.g., a peptide map of a recombinant protein), the selectivity of peptide separations can be optimized by choice and concentration of acidic modifier, without compromising the ability to obtain effective sequence coverage of a protein. In some cases, these selectivity differences were explored further, and a rational basis for differentiating acidic modifier effects from the underlying peptide sequences is described.
科研通智能强力驱动
Strongly Powered by AbleSci AI