增强子
聚腺苷酸
生物
发起人
基因
氯霉素乙酰转移酶
遗传学
序列(生物学)
乳腺腺病毒
分子生物学
基因表达
腺病毒科
重组DNA
作者
Wendy Sheay,Stefanie A. Nelson,I. Martínez,Tingting Chu,Smita Bhatia,Alex Dornburg
出处
期刊:PubMed
日期:1993-11-01
卷期号:15 (5): 856-62
被引量:17
摘要
A series of universal eukaryotic gene expression vectors was constructed. All vectors contain a viral promoter and enhancer, a polylinker for insertion of the gene of interest and a polyadenylation sequence. To enhance translation, we inserted the tripartite leader sequence of an adenovirus downstream of the promoter. Using the chloramphenicol acetyl transferase (cat) gene as a marker, we show that the strength of various promoters/enhancers in different cell lines differed by two orders of magnitude. The presence of the tripartite leader increased the efficiency of gene expression up to 18-fold. The level of increase is promoter specific and is most likely influenced by additional sequences flanking the tripartite leader sequence.
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