甲基化DNA免疫沉淀
微阵列
CpG站点
甲基化
DNA甲基化
照明菌甲基化试验
分子生物学
生物
DNA微阵列
DNA
计算生物学
遗传学
基因
基因表达
作者
Hilary A. A. Colyer,Margaret Dellett,Ken I. Mills
出处
期刊:Methods in molecular biology
日期:2012-01-01
卷期号:: 329-339
被引量:2
标识
DOI:10.1007/978-1-61779-612-8_21
摘要
Methylated CpG island amplification and microarray (MCAM) is a two-color array technique that quantifies DNA methylation by hybridizing equimolar amounts of treated vs. control cell-line DNA to an array platform. Sample preparation, hybridization, and scanning are performed over 1 week, but multiple samples can be prepared simultaneously, allowing for high-throughput processing and data acquisition. One microarray slide is required for each sample assayed, with a control sample hybridized with each test sample.
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