ISOLATION OF FLUORESCENT PSEUDOMONAS FROM SOIL, MEDIUM OPTIMIZATION AND DESIGNING OF DIAGNOSTIC MEDIA.

The isolation of the Fluorescent Pseudomonas spp .from soil is difficult and therefore the present study is focused on the isolation , identification and the optimum requirements for its growth and pigment production. The biosynthesis of yellow green fluorescent water soluble pigment by Pseudomonas spp. occurs under iron deficient conditions .The isolated organism Pseudomonas aeruginosa JCM 5962T shows the presence of two types of secondary metabolite pigments: Pyocyanin and Pyoverdine .The antagonistic property of organism is due to Pyocyanin whereas pyoverdine is a siderophore which forms stable Fe III complex and fluoresces under UV light.By a simple modification common commercial dehydrated laboratory media can be used for the evaluation of fluorescent pigment excretion by Pseudomonas. The addition of sufficient sterile Egg white or iron binding egg white protein Albumin to bind all the iron in these media converted them to efficient diagnostic media .The absorption maxima of pyoverdine were obtained at 405 nm whereas pyocyanin absorbs more at 520 nm.