Production of Osteopontin and Osteocalcin by MG63 Cells In Response to Bone Morophogenetic Proteins and IGF‐1

Bone morphogenetic proteins (BMPs) are a group of structurally related proteins in the transforming growth factor‐beta (TGF‐beta) family which have been shown to stimulate bone formation in vivo. The hypothesis of this study was that osteoblast‐like cells (OBs) treated with BMP would have similar levels of secretion of osteopontin (OPN) and osteocalcin (OCN) to that of OBs treated with IGF‐1. Specifically, the aims of this study were to evaluate osteoblast‐like cells (MG‐63 cell line) for secretion of both OPN and OCN after treatment with DBM, OP‐1, or insulin‐like growth factor‐1 (IGF‐1) compared to control, at 24, 48, and 72 hours. After each incubation period, ELISA kits were used to determine the levels of osteopontin and osteocalcin production. The results clearly demonstrated a rise of OCN at 48 hours and a fall at 72 hours for all samples, including control groups. However, there was no significant difference between groups (p > 0.05). With the OPN assay, results showed no significant difference between groups until 72 hours (p = 0.022), where the IGF‐1 group was significantly higher than the Control and (p = 0.021, respectively). This information is important for understanding the signaling pathways that may be innervated in the osteoblast following stimulation with growth factors.