Role of Yoga and Its Plausible Mechanism in the Mitigation of DNA Damage in Type-2 Diabetes: A Randomized Clinical Trial

Abstract Background Although yoga is found to be beneficial in the management of type 2 diabetes (T2D), its mechanism of action is poorly understood. T2D is also known to be associated with increased oxidative stress (OS) and DNA damage. Purpose This study examines how yoga modulates OS-induced DNA damage and the efficiency of DNA repair in T2D conditions. Methods In this assessor-masked randomized clinical trial, T2D subjects (n = 61), aged (Mean ± SD, 50.3 ± 4.2) were randomly allocated into Yoga group (31) that received 10 weeks of yoga intervention and Control (30) with routine exercises. Molecular and biochemical assessments were done before and after the intervention period. Structural Equation Modeling using “R” was used for mediation analysis. Results At the end of the 10th week, Yoga group showed significant reduction in DNA damage indicators like Tail Moment (−5.88[95%CI: −10.47 to −1.30]; P = .013) and Olive Tail Moment (−2.93[95%CI: −4.87 to −1.00]; P < .01), oxidative DNA damage marker 8-OHdG (−60.39[95%CI: −92.55 to −28.23]; P < .001) and Fasting Blood Sugar (-22.58[95%CI: −44.33 to −0.83]; P = .042) compared to Control. OGG1 protein expression indicating DNA repair, improved significantly (17.55[95%CI:1.37 to 33.73]; P = .034) whereas Total Antioxidant Capacity did not (5.80[95%CI: -0.86 to 12.47]; P = 0.086). Mediation analysis indicated that improvements in oxidative DNA damage and DNA repair together played a major mediatory role (97.4%) in carrying the effect of yoga. Conclusion The beneficial effect of yoga on DNA damage in T2D subjects was found to be mediated by mitigation of oxidative DNA damage and enhancement of DNA repair. Clinical Trial information (www.ctri.nic.in) CTRI/2018/07/014825