大肠杆菌
细菌
吲哚试验
酶
生物化学
微生物
化学
细胞内
功能(生物学)
转基因生物
基因工程
微生物学
生物
基因
细胞生物学
遗传学
作者
Lingbin Lu,Yanjun Liu,Gong Zhang,Yun Ge,Xinyuan Fan,Lin Feng,Jie Wang,Huangtao Zheng,Xiao Xie,Xiangmei Zeng,Peng R. Chen
出处
期刊:Biochemistry
[American Chemical Society]
日期:2017-11-29
卷期号:57 (4): 446-450
被引量:25
标识
DOI:10.1021/acs.biochem.7b01017
摘要
We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to the metabolically labile photocaging groups inside Escherichia coli, our chemical decaging strategy that relies on the inverse electron-demand Diels-Alder (iDA) reaction is compatible with the intracellular environment of bacteria, which can be a general tool for gain-of-function study of a given protein in prokaryotic systems. By applying this strategy for in situ activation of the indole-producing enzyme TnaA, we built an orthogonal and chemically inducible indole production pathway inside E. coli cells, which revealed the role of indole in bacterial antibiotic tolerance.
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