低温保存
间质细胞
男科
活力测定
胸腺切除术
移植
生物
基质
人口
免疫学
细胞
医学
细胞生物学
外科
重症肌无力
癌症研究
生物化学
胚胎
环境卫生
免疫组织化学
作者
Valentin P. Shichkin,Олександр Горбач,Olga A. Zuieva,Nataliia I. Grabchenko,Irina A. Aksyonova,Boris M. Todurov
出处
期刊:Cryobiology
[Elsevier]
日期:2017-10-01
卷期号:78: 70-79
被引量:9
标识
DOI:10.1016/j.cryobiol.2017.06.010
摘要
The thymus is the major site of T lymphocyte generation and so is critical for a functional adaptive immune system. Since, thymectomy is a component of neonatal surgery for congenital heart diseases, it provides great potential for collection and storage of thymic tissue for autologous transplantation. However, specific investigation into the optimum parameters for thymic tissue cryopreservation have not been conducted. In this research, we evaluated the effect of different cryoprotective media compositions, which included penetrating (Me2SO, glycerol) and non-penetrating (dextran-40, sucrose, hydroxyethyl starch) components, on the viability and functionality of frozen-thawed human thymic samples to select an optimal cryoprotective medium suitable for long-term storage of thymic tissue and a stromal-epithelial enriched population. Our primary focus was on receiving, low-temperature storage, culturing and evaluation of thymic tissue samples from newborns and infants with congenital heart diseases, who had undergone thymectomy as a part of standard surgical procedure. Thus, this work builds the platform for autologous clinical intervention into the thymus-deficient patients with congenital heart diseases. From our data, we conclude that although there were no significant differences in efficiency of tested cryoprotective media compositions, the combination of Me2SO and dextran-40 compounds was the most suitable for long-term storage both thymic cell suspensions and thymic fragments based on the viability of CD326+ epithelial cells and stromal-epithelial cell monolayer formation.
科研通智能强力驱动
Strongly Powered by AbleSci AI