背景(考古学)
分离(微生物学)
髓系细胞
生物
同种类的
免疫学
癌症
抗体
人口
髓样
细胞生物学
微生物学
医学
遗传学
热力学
环境卫生
物理
古生物学
作者
Jitka Yehudith Sagiv,Sandra Voels,Zvi Granot
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 179-193
被引量:29
标识
DOI:10.1007/978-1-4939-3801-8_13
摘要
Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.
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