Immunoprecipitation for Cell Culture

Immunoprecipitation (IP) is a method to pull down a protein out of solution using an antibody that specifically binds to that particular protein. Immunoprecipitation is a powerful technique to isolate and concentrate a particular protein from a sample containing many thousands of different proteins, to test protein-protein interactions, and to pull multiple members of a complex out of solution by latching onto one member with an antibody. This protocol describes a general immunoprecipitation strategy using cell cultures as starting material.