The CBL-interacting protein kinase CaCIPK13 positively regulates defence mechanisms against cold stress in pepper

胡椒粉 生物化学 冷应激 激酶 蛋白激酶A 细胞生物学 化学 生物 园艺 基因
作者
Xiao Ma,Wen-Xian Gai,Yang Li,Yanan Yu,Muhammad Ali,Zhen‐Hui Gong
出处
期刊:Journal of Experimental Botany [Oxford University Press]
卷期号:73 (5): 1655-1667 被引量:32
标识
DOI:10.1093/jxb/erab505
摘要

Abstract Cold stress is one of the main factors limiting growth and development in pepper. Calcineurin B-like proteins (CBLs) are specific calcium sensors with non-canonical EF-hands to capture calcium signals, and interact with CBL-interacting protein kinases (CIPKs) in the regulation of various stresses. In this study, we isolated a cold-induced CIPK gene from pepper named CaCIPK13, which encodes a protein of 487 amino acids. In silico analyses indicated that CaCIPK13 is a typical CIPK family member with a conserved NAF motif, which consists of the amino acids asparagine, alanine, and phenylalanine. The CaCIPK13 protein was located in the nucleus and plasma membrane. Knock down of CaCIPK13 resulted in enhanced sensitivity to cold stress in pepper, with increased malondialdehyde content, H2O2 accumulation, and electrolyte leakage, while the catalase, peroxidase, superoxide dismutase activities and anthocyanin content were decreased. The transcript level of cold and anthocyanin-related genes was substantially decreased in CaCIPK13-silenced pepper leaves relative to the empty vector control. On the contrary, overexpression of CaCIPK13 in tomato improved cold tolerance via increasing anthocyanin content and activities of reactive oxygen species scavenging enzymes. Furthermore, the interaction of CaCIPK13 with CaCBL1/6/7/8 was Ca2+-dependent. These results indicate that CaCIPK13 plays a positive role in cold tolerance mechanism via CBL-CIPK signalling.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
ZZQ发布了新的文献求助10
1秒前
郭大壮完成签到,获得积分20
2秒前
无花果应助a东采纳,获得10
3秒前
3秒前
nxy完成签到 ,获得积分10
4秒前
科目三应助yannick采纳,获得10
4秒前
7秒前
yxq完成签到,获得积分10
7秒前
8秒前
彭于晏应助跳跃雅青采纳,获得10
8秒前
8秒前
Gaowenjie发布了新的文献求助10
9秒前
ZZQ完成签到,获得积分10
9秒前
a东应助文件撤销了驳回
9秒前
小情绪完成签到,获得积分0
9秒前
11秒前
贪玩半仙完成签到 ,获得积分10
13秒前
燕小丙完成签到,获得积分10
15秒前
牛无施发布了新的文献求助10
15秒前
传奇3应助科研通管家采纳,获得10
16秒前
科研通AI2S应助科研通管家采纳,获得10
16秒前
今后应助科研通管家采纳,获得10
16秒前
浮游应助科研通管家采纳,获得10
16秒前
慕青应助科研通管家采纳,获得10
16秒前
浮游应助科研通管家采纳,获得10
16秒前
科研通AI2S应助科研通管家采纳,获得10
16秒前
科研通AI5应助科研通管家采纳,获得10
16秒前
lilili应助科研通管家采纳,获得10
16秒前
英俊的铭应助科研通管家采纳,获得10
16秒前
16秒前
16秒前
天天快乐应助科研通管家采纳,获得10
16秒前
19秒前
19秒前
zsp完成签到,获得积分10
22秒前
从容荠发布了新的文献求助10
24秒前
君君发布了新的文献求助10
24秒前
LL完成签到,获得积分10
25秒前
25秒前
俊秀的芫发布了新的文献求助10
28秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Acute Mountain Sickness 2000
Handbook of Milkfat Fractionation Technology and Application, by Kerry E. Kaylegian and Robert C. Lindsay, AOCS Press, 1995 1000
A novel angiographic index for predicting the efficacy of drug-coated balloons in small vessels 500
Textbook of Neonatal Resuscitation ® 500
The Affinity Designer Manual - Version 2: A Step-by-Step Beginner's Guide 500
Affinity Designer Essentials: A Complete Guide to Vector Art: Your Ultimate Handbook for High-Quality Vector Graphics 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 内科学 生物化学 物理 计算机科学 纳米技术 遗传学 基因 复合材料 化学工程 物理化学 病理 催化作用 免疫学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 5069715
求助须知:如何正确求助?哪些是违规求助? 4290967
关于积分的说明 13369157
捐赠科研通 4111230
什么是DOI,文献DOI怎么找? 2251352
邀请新用户注册赠送积分活动 1256540
关于科研通互助平台的介绍 1189031