A review on physico-chemical delignification as a pretreatment of lignocellulosic biomass for enhanced bioconversion

Effective pretreatment of lignocellulosic biomass (LCB) is one of the most important steps in biorefinery, ensuring the quality and commercial viability of the overall bioprocess. Lignin recalcitrance in LCB is a major bottleneck in biological conversion as the polymerization of lignin with hemicellulose hinders enzyme accessibility and further bioconversion to fuels and chemicals. Therefore, there is a need to delignify LCB to ease further bioprocessing. The efficiency of delignification, quality and quantity of the desired products, and generation of inhibitors depend upon the type of pretreatment employed. This review summarizes different single and integrated physicochemical pretreatments for delignification. Additionally, conditions required for effective delignification and the advantages and drawbacks of each method were evaluated. Advances in overcoming the recalcitrance of residual lignin to saccharification and the methods to recover lignin after delignification are also discussed. Efficient lignin recovery and valorization strategies provide an avenue for the sustainable lignocellulose biorefinery.