纤维素酶
化学
水解
木质纤维素生物量
生物量(生态学)
固定化酶
纤维素
酶水解
化学工程
生物燃料
色谱法
制浆造纸工业
有机化学
生物技术
酶
农学
生物
工程类
作者
Shah Samiur Rashid,Abu Hasnat Mustafa,Mohd Hasbi Ab. Rahim,Burcu Gunes
标识
DOI:10.1016/j.ijbiomac.2022.04.072
摘要
In this research, a magnetic reusable nickel nanoparticle (NiNPs) supporting materials were prepared for cellulase enzyme immobilization. The immobilized cellulase showed high activity recovery, large & fast immobilization capacity and improved pH & temperature tolerance. The excellent stability and reusability enabled the immobilized cellulase to retain 84% of its initial activity after ten cycles. At 2 mg/mL enzyme concentration, highest 93% immobilization efficiency was achieved within two hours of immobilization. When the treatment temperature reached 40 °C and pH 5, the immobilized cellulase exhibited highest residual activity. The immobilized cellulase could be separated from the solution by a magnetic force. This study introduced a novel supporting material for cellulase immobilization, and the immobilized cellulase poses a great potential in the hydrolysis of lignocellulosic biomass which can used as an easily applicable and sustainable pre-treatment step for advanced biofuel production.
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