An optimization by response surface methodology for the enhanced production of rMBSP from Pichia pastoris and study of its application

GS115 in our laboratory. However, low production of rMBSP in shake flask constraints further exploration of properties. A 5-L high cell density fermentation was performed and the fermentation medium was optimized. Response surface methodology (RSM) was used to optimize the culture condition through modeling three selected parameter. for rMBSP. LC-MS/MS analysis demonstrated the specific cleavage of bovine serum albumin by rMPSP. These findings suggest that rMPSP has potential as a valuable enzyme for protein science research.