Development of a label-free impedimetric aptasensor based on Zr-MOF and titaniom carbide nanosheets for detection of L-Tryptophan

This study primarily focuses on the L-Tryptophan (Trp) biomarker assay, with particular attention given to its objectives. The investigation centers on the potential implications of imbalanced Trp levels and its associated metabolites, which have been attributed to the spectrum of both psychological and physiological disorders, encompassing conditions such as cancer. Therefore, the swift and accurate detection of this amino acid is of paramount importance in cancer monitoring, as it plays a crucial role in preventing the metastasis and spread of cancer cells. Thus, an electrochemical aptasensor was designed based on nanocomposite of AgNPs@UiO-66/Ti3C2 (MXene) as immobilization strategy for the ultrasensitive detection of Trp. Zirconium metal–organic frameworks (Zr-MOFs) were employed as carriers for silver nanoparticles to facilitate subsequent binding with aptamers. Markedly, the obtained results show that the constructed aptasensor can specifically detect Trp in the two concentration range from 1fM to 1 nM and 1 nM to 200 nM and with a low detection limit of 0.35 fM. When applied to real samples, the experimental results demonstrated positive recovery rates. Consequently, a discerning and sensitive electrochemical aptasensor devoid of labeling agents was successfully fabricated for the explicit purpose of Trp detection.