生物
Lasso(编程语言)
基因
膀胱癌
癌症研究
接收机工作特性
核糖体蛋白
计算生物学
生物信息学
肿瘤科
遗传学
癌症
内科学
医学
核糖核酸
核糖体
万维网
计算机科学
作者
Cheng Li,Zhengqiang Wan,Qiuhua Deng,Zhigang Li,Yinglei Wang
出处
期刊:Archivos españoles de urología
[SciELO]
日期:2023-01-01
卷期号:76 (8): 605-605
标识
DOI:10.56434/j.arch.esp.urol.20237608.75
摘要
Background: The aim of this study was to investigate the common gene signatures and potential molecular mechanisms of bladder urothelial carcinoma (BLCA) and metabolic syndrome (MS). Methods: Transcriptome data for BLCA and MS were obtained from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was utilized to identify co-expression networks associated with BLCA and MS, and five hub genes were further screened and validated using logistic least absolute shrinkage and selection operator (LASSO) regression models and receiver operating characteristic (ROC) curve, and external dataset for validation. The relationship between the hub genes and the clinicopathological characteristics and prognosis of BLCA patients was explored in the GEO and The Cancer Genome Atlas (TCGA)-BLCA cohorts, respectively. Differences in the immune microenvironment of BLCA and MS were analyzed using the database CIBERSORT and the R package “ssGSEA”, and the correlation between hub genes and tumor microenvironment, immune score and targeted drugs was analyzed with the help of the TCGA-BLCA cohort. Finally, BLCA single-cell RNA (scRNA) data were used to analyze the expression levels of the hub genes in various cell types of BLCA and molecular mechanisms. Results: Five hub genes were screened by WGCNA and LASSO regression analysis, namely AP2-associated protein kinase 1 (AAK1), ATP-binding cassette subfamily F member 2 (ABCF2), Mitochondrial ribosomal protein L42 (MRPL42), La-related protein 3 (SSB) and TATA-box binding protein-associated factor 10 (TAF10). Analyzed in the GEO and TCGA-BLCA cohorts, we found that the hub genes (TAF10 and ABCF2) were closely associated with the clinicopathological characteristics and prognosis of BLCA patients. In CIBERSORT, we discovered that the hub genes are closely linked to the immune microenvironment, immune score, and especially with dendritic cells (DCs). In the single-cell RNA sequencing (scRNA-seq) analysis of BLCA, we identified that SSB was significantly differentially expressed in BLCA and normal bladder tissues and that it plays an important role in the development of BLCA. Conclusions: The interaction of BLCA with MS may be associated with several cancer pathways being activated and identified TAF10 and ABCF2 as potential biomarkers and therapeutic targets for patients with BLCA and MS.
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