抗坏血酸
脱氢抗坏血酸
荧光
化学
氧化酶试验
纳米颗粒
检出限
基质(水族馆)
核化学
酶
材料科学
纳米技术
生物化学
色谱法
生物
物理
生态学
量子力学
食品科学
作者
Juan Huang,Chenyang Shen,Hongfei Gu,Wang Guo,Peng Zhou,Xinyu Liu,K. W. Yu,Yuanlong Qin,Kang Zhou,Jiayue Zhang,Zhengbo Chen
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2023-11-30
卷期号:11 (49): 17472-17481
标识
DOI:10.1021/acssuschemeng.3c05519
摘要
Although compared with natural enzymes and traditional artificial enzymes, oxidase mimetic nanoenzymes have superior performance; the number of specific biomolecules as the substrate is minimal, especially for ascorbic acid (AA) oxidase nanoenzymes. Herein, Cu nanoparticles/N-doped Ti3C2Tx (Cu NPs/N–Ti3C2Tx), containing copper mimicking the active sites of natural AA oxidase, exhibit specific AA oxidase-like catalytic activity. Dehydroascorbic acid (DHAA) obtained from the oxidation of AA can react with o-phenylenediamine (OPD) to produce fluorescent 3-(1,2-dihydrox-yethyl) furo[3,4-b]quinoxaline-1-one (DFQ). Thus, a fluorescence AA sensor is established based on the AA oxidase mimetic activity of Cu NPs/N–Ti3C2Tx. Remarkably, an excellent linear range of 5–150 μM for the fluorescence method with a low detection limit of 0.437 μM could guarantee the sensitive detection of AA. In addition, the synthesized Cu NPs/N–Ti3C2Tx can induce the generation of reactive oxygen species by accelerating the oxidation of AA, thereby effectively inhibiting the proliferation of cancer cells.
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