纳米结构
纳米技术
DNA
DNA折纸
材料科学
DNA纳米技术
自组装
化学
生物物理学
生物
生物化学
作者
Qinyi Lu,Yang Xu,Erik Poppleton,Kun Zhou,Petr Šulc,Nicholas Stephanopoulos,Yonggang Ke
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-01-26
标识
DOI:10.1021/acs.nanolett.3c04497
摘要
The development of methods to synthesize artificial protein complexes with precisely controlled configurations will enable diverse biological and medical applications. Using DNA to link proteins provides programmability that can be difficult to achieve with other methods. Here, we use DNA origami as an "assembler" to guide the linking of protein-DNA conjugates using a series of oligonucleotide hybridization and displacement operations. We constructed several isomeric protein nanostructures, including a dimer, two types of trimer structures, and three types of tetramer assemblies, on a DNA origami platform by using a C3-symmetric building block composed of a protein trimer modified with DNA handles. Our approach expands the scope for the precise assembly of protein-based nanostructures and will enable the formulation of functional protein complexes with stoichiometric and geometric control.
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