生物
基因组
进化生物学
基因组大小
倍性
杂合子丢失
遗传学
基因
等位基因
作者
My‐Hanh Le,Brett Morgan,Mei-Yeh Jade Lu,Víctor Moctezuma,Oscar Burgos,Jen‐Pan Huang
标识
DOI:10.1111/1755-0998.13908
摘要
Abstract Beetles, despite their remarkable biodiversity and a long history of research, remain lacking in reference genomes annotated with structural variations in loci of adaptive significance. We sequenced and assembled high‐quality chromosome‐level genomes of four Hercules beetles which exhibit divergence in male horn size and shape and body colouration. The four Hercules beetle genomes were assembled to 11 pseudo‐chromosomes, where the three genomes assembled using Nanopore data ( Dynastes grantii , D. hyllus and D. tityus ) were mapped to the genome assembled using PacBio + Hi‐C data ( D. maya ). We demonstrated a striking similarity in genome structure among the four species. This conservative genome structure may be attributed to our use of the D. maya assembly as the reference; however, it is worth noting that such a conservative genome structure is a recurring phenomenon among scarab beetles. We further identified homologues of nine and three candidate‐gene families that may be associated with the evolution of horn structure and body colouration respectively. Structural variations in Scr and Ebony2 were detected and discussed for their putative impacts on generating morphological diversity in beetles. We also reconstructed the demographic histories of the four Hercules beetles using heterozygosity information from the diploid genomes. We found that the demographic histories of the beetles closely recapitulated historical changes in suitable forest habitats driven by climate shifts.
科研通智能强力驱动
Strongly Powered by AbleSci AI