莱菔硫烷
排泄
代谢物
萝卜硫苷
尿
代谢组
生物
粪便
新陈代谢
肠道菌群
化学
食品科学
生物化学
植物
硫代葡萄糖苷
微生物学
芸苔属
作者
John A. Bouranis,Laura M. Beaver,Carmen P. Wong,Jaewoo Choi,Sean Hamer,Edward W. Davis,Kevin Brown,Duo Jiang,Thomas J. Sharpton,Jan F. Stevens,Emily Ho
标识
DOI:10.1002/mnfr.202300286
摘要
The glucosinolate glucoraphanin from broccoli is converted to sulforaphane (SFN) or sulforaphane-nitrile (SFN-NIT) by plant enzymes or the gut microbiome. Human feeding studies typically observe high inter-individual variation in absorption and excretion of SFN, however, the source of this variation is not fully known. To address this, a human feeding trial to comprehensively evaluate inter-individual variation in the absorption and excretion of all known SFN metabolites in urine, plasma, and stool, and tested the hypothesis that gut microbiome composition influences inter-individual variation in total SFN excretion has been conducted.Participants (n = 55) consumed a single serving of broccoli or alfalfa sprouts and plasma, stool, and total urine are collected over 72 h for quantification of SFN metabolites and gut microbiome profiling using 16S gene sequencing. SFN-NIT excretion is markedly slower than SFN excretion (72 h vs 24 h). Members of genus Bifidobacterium, Dorea, and Ruminococcus torques are positively associated with SFN metabolite excretion while members of genus Alistipes and Blautia has a negative association.This is the first report of SFN-NIT metabolite levels in human plasma, urine, and stool following consumption of broccoli sprouts. The results help explain factors driving inter-individual variation in SFN metabolism and are relevant for precision nutrition.
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