碘化丙啶
膜
材料科学
电场
离子
生物物理学
电压
泄漏(经济)
纳米技术
化学
电气工程
生物化学
物理
生物
细胞凋亡
程序性细胞死亡
有机化学
经济
宏观经济学
工程类
量子力学
作者
Venkata Rao Krishnamurthi,Ariel Rogers,Janet Peifer,Isabelle I. Niyonshuti,Jingyi Chen,Yong Wang
标识
DOI:10.1101/2020.03.13.991067
摘要
Abstract Physical agents such as low electric voltages and currents have recently gained attention for antimicrobial treatment due to their bactericidal capability. Although microampere electric currents were shown to suppress the growth of bacteria, it remains unclear to what extent the microampere currents damage bacterial membrane. Here, we investigated the membrane damage and two-way leakage caused by microampere electric currents (≤ 100 μA) in a short time (30 min). Based on MitoTracker staining, propidium iodide staining, filtration assays, and quantitative single-molecule localization microscopy, we found that microampere electric currents caused significant membrane damages and allowed two-way leakages of ions, small molecules and proteins. This study paves the way to new development and antibiotic applications of ultra-low electric voltages and currents. Statement of Significance Previous studies showed that treating bacteria with milliampere electric currents for 72 hours led to significant damages of the bacterial membrane. However, it remains unclear to what extent membrane damages and two-way (i.e. inward and outward) leakages are caused by lower electric currents in a shorter time. In this work, we set out to answer this question. We carried out several assays on the bacteria treated by microampere electric currents of ≤ 100 μA for 30 min, including MitoTracker staining, propidium iodide staining, filtration assays, and quantitative single-molecule localization microscopy. We found and quantified that the membrane damages were caused by microampere electric currents in half an hour and allowed two-way leakages of ions, small molecules, and proteins.
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