The molecular mechanisms of silica nanomaterials enhancing the rice (Oryza sativa L.) resistance to planthoppers (Nilaparvata lugens Stal)

Herein, fluorescent silica (F-SiO2) ENMs (50 nm) were synthesized, which could be taken up and translocated from rice root to shoot, promoting the plant growth and resistance to planthopper compared with Si ion fertilizers under hydroponic conditions. Particularly, upon exposure F-SiO2 ENMs (5 mg‧L−1) suspension for 9 days, the fresh and dry weight (FW and DW) of shoot, the root length, surface area, and tip number were increased by 33.58%, 65.22%, 15.26%, 20.26% and 29.01%, respectively. Notably, in the presence of planthopper, the shoot FW and DW still increased by 61.88% and 114.75%, respectively. The increased lignin content (by 30.13%) and formation of silica cells in stem after F-SiO2 ENMs exposure (5 mg‧L−1) could be mechanical barriers against planthoppers. The transcriptome data revealed that F-SiO2 ENMs could upregulate the expression of genes involved in plant-pathogen interactions, plant hormone signal transduction, glucose metabolism and carbon fixation pathway, promoting the growth and resistance of rice seedlings. Our findings provide first evidence for the underlying molecular mechanisms of SiO2 ENMs enhancing the rice resistance to planthopper.