重编程
转分化
生物
细胞生物学
表观遗传学
转录因子
体细胞
诱导多能干细胞
细胞命运测定
计算生物学
细胞效价
细胞分化
干细胞
细胞
遗传学
胚胎干细胞
基因
作者
Hao Fei Wang,Yuchen Yang,Jiandong Liu,Qian Li
标识
DOI:10.1038/s41580-021-00335-z
摘要
The reprogramming of somatic cells with defined factors, which converts cells from one lineage into cells of another, has greatly reshaped our traditional views on cell identity and cell fate determination. Direct reprogramming (also known as transdifferentiation) refers to cell fate conversion without transitioning through an intermediary pluripotent state. Given that the number of cell types that can be generated by direct reprogramming is rapidly increasing, it has become a promising strategy to produce functional cells for therapeutic purposes. This Review discusses the evolution of direct reprogramming from a transcription factor-based method to a small-molecule-driven approach, the recent progress in enhancing reprogrammed cell maturation, and the challenges associated with in vivo direct reprogramming for translational applications. It also describes our current understanding of the molecular mechanisms underlying direct reprogramming, including the role of transcription factors, epigenetic modifications, non-coding RNAs, and the function of metabolic reprogramming, and highlights novel insights gained from single-cell omics studies.
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