Application of Tissue Microarray and Immunohistochemistry Technique to the Diagnosis of Endometrial Cancer

Objective To evaluate the significance of the appliance of tissue microarray and immunohistochemistry technique on detecting the gene expression in endometrial cancer.Methods The expressions of P16,PTEN,E-cadherin and CtBP1 of the 60 patients with endometrial cancer(experimental group) and 30 patients with normal endometrial(control group) were observed by tissue microarray technology and immunohistochemichal methods.Results The Positive expressions of P16,PTEN and E-cadherin of the patients in the experimental group were significantly higher than those in the control group(P 0.05),while there was no difference of CtBP1 expression between the two groups(P 0.05) .Besides,there were no differences of the expressions of 4 indicators of immunohistochemistry in different cancer cell differentiation(P 0.05) .Conclusion Tissue microarray and immunohistochemistry technique could be useful and reliable in the quick diagnosis of endometrial cancer in the mass samples,which indicates that there are relationships between P16,PTEN,E-cadherin and endometrial cancer and they are of reference values for pathologic diagnosis.