Simultaneous Assay of Imperatorin and Isoimperatorin in Shizen Ointment by QAMS

Objective:To develop a method for simultaneously determining imperatorin and isoimperatorin in Shizen ointment by single marker.Method: Using imperatorin as the internal reference substance,the relative correction factor(RCF) of isoimperatorin was determined by HPLC on different instruments and columns,with good reproducibility.The content of imperatorin in 10 batches of ointment was authentically determined by the external standard method,and the content of isoimperatorin was calculated by external standard method and RCF,respectively.Result: No siginificant differences between the quantitative results of QAMS method and external standard method were observed.Conclusion: QAMS is feasible for determination of imperatorin and isoimperatorin simultaneously,and the developed method can be used for quality control of Shizhen ointment.