Study of the cyclic peptide binding to HIV-1 gp41 NHR

Objective To find small molecular that can lead to inhibition of HIV-1 infection in early stage by screening the cyclic peptide that can bind to HIV-1 gp41 NHR from phage display peptide library.Methods C7C phage displayed peptide library was biopanning by(using) the P+LS method with target molecule of N36 peptide derived from the gp41 NHR.Phage clones were identified by ELISA.Based on the DNA sequence of positive clones,the cyclic peptide was synthesized.The binding between N36 and the cyclic peptide was identified.Re-(sults After) 3 rounds of screening,11 phage clones were identified as the positive clones.All these clones shared a unique amino acid sequence—CDRHQHKRC,which was named NA peptide.The modified NA peptide(Biotin-SACDRHQHKRCGG) was synthesized and conju-(gated) with BSA as carrier.The binding between NA-BSA conjugate and peptide N36 was blocked by soluble peptides N36 and C34 (derived from gp41 CHR).Conclusion The cyclic peptide SACDRHQHKRCGG is the HIV-1 gp41 NHR binding peptide.