In order to explore community characteristics and distribution of aquatic macrophytes in Henan section of Huaihe River Basin and the causes,field survey and in-laboratory analysis were carried out of composition of aquatic macrophytes,and characteristics of the water and the soil environment and their relationship in the basin. Results show that a total of 51 species of aquatic macrophytes were detected,including 28 species of emergent plants,12 species of submerged plants and 10 species of floating-leaved plants,1 species of floating plant,which accounts for 54. 9%,23. 5%,19. 6%and 2. 0% of the total,respectively. They formed 14 types of communities,of which 5 were dominated with submerged plants,6 with emergent plants and 3 with floating leaf plants. The communities varied sharply in environment,especially factors,like NH4+-N,NO3--N,TN,TP and PO43-in the water. CCA reveals that water environment factors,such as TP,PO43-,NH4+-N and DO concentrations,were the key factors determining distribution of the aquatic macrophytes in the Huaihe River Basin.