Real-time Fluorescence PCR Method for the Detection of Citrus Component in Fruit Juice

A real-time fluorescence polymerase chain reaction method using the fluorescence dye SYBR Green and aimed at citrus trnL gene sequence was established for detection of citrus DNA in fruit juice. The citrusspecific primers were designed according to the sequence alignment result between 20 different citrus trnL gene sequences. The citrus component could be detected by the citrus-specific primers with a limit of detection of 0.1 pg/μL DNA. The following detection of 10 fruit juice and beverage samples showed that this method was suitable to identify the citrus component in fruit juice beverage and other relative food and can provide a technique base for research of fruit juice adulteration detection.