Study on gold immunochromatography assay for rapid detection of Trimethoprim

To establish a rapid assay for detection of Trimethoprim(TMP),gold immunochromatography assay(GICA) was studied.Colloidal gold marked coupled with monoclonal antibody against Trimethoprim was jet sprayed onto the glass fiber.TMP-OVA and anti-mouse goat immunoglobulins were jet-positioned onto a nitrocellulose membrane.Sample pad,gold jed pad,nitrocellulose membrane and absorbent paper were assembled and cut into detecting card,respectively.The results showed that the sentitive of rapid strip for TMP visual detection limit was 50 μg/L and detection time within 5 min.Repeatability of strip is good,and the assay gives no false positive and false negative results compared with ELISA and LC-MS/MS.So it is very simple and convenient to rapidly detect TMP on the spot.