Production of multi-enzyme enriched bio-feed from camellia oleifera seed cake in solid state fermentation by Aspergillus niger

In this paper,Aspergillus niger was used to produce multi-enzyme enriched bio-feed from camellia oleifera seed cake with solid-state fermentation.The suitable medium for mixed fermentation of Aspergillus niger were soya bean meal to oil-tea-cake ratio of 1:1,material-to-water ratio of 1: 0.5,1%(W/W)(NH4)2SO4,0.1%(W/W) K2HPO4,and 50 g dry material in 500 mL tapered bottle.The best fermentation conditions were 1.5%(W/W) inoculum size of Aspergillus niger,at 28℃ for 4 d at pH 5.The activities of proteinase and cellulase could reach to 4 128.1 U?g-1 and 1 481.9 U?g-1,respectively.The crude protein and the pure protein in camellia oleifera seed cake increased by 41.8%,48.2%,respectively,while the crude fibre,tannin and tea saponin reduced by 38.0%,78.7%,and 65.3%,respectively.Nutritional values of the fermented bio-feed improve obviously.