Immunofluorescence Staining of WT-1/Podocalyxin on Mouse Kidney Sections

In glomerular diseases, podocytes are one type of cells involved in dysfunction of glomerular filtration.In these conditions, podocyte proteins expression change.Therefore, immunofluorescent staining of podocytes can be performed to visualize podocyte localization of proteins of interest.In this protocol, we detail a method to stain podocytes with a specific marker WT-1 (Wilms Tumor-1) for "in situ" podocyte analysis by immunofluorescent microscopy, more informative technique than other techniques (as Western blot).