Multi-Scale Optical Molecular Imaging

In this talk I will present an array of fluorescence molecular imaging technologies for multi-scale imaging from microscopic to macroscopic scales. Specific methods include planar fluorescence imaging for macroscopic samples, fluorescence laminar optical tomography (LOT) for mesoscopic (millimeter scale) samples, and two-photon fluorescence microscopy for intravital microscopic imaging. In addition, needle-based endoscopic imaging of deep tissues will be presented. Specific biomedical examples will include in vivo imaging of renal and brain function, 3D imaging of stem cell migration in scaffolds, and molecular imaging of cancer. Lastly, I will introduce our ongoing efforts in collaboration with FDA scientists on the development of standard testing methods for evaluation of optical molecular imaging devices.