核小体
染色质重塑
染色质
组蛋白八聚体
组蛋白
DNA
生物
细胞生物学
染色质结构重塑复合物
DNA测序
组蛋白密码
遗传学
瑞士/瑞士法郎
作者
Karsten Rippe,Anna Schrader,Philipp Riede,Ralf Strohner,Elisabeth Lehmann,Gernot Längst
标识
DOI:10.1073/pnas.0702430104
摘要
Chromatin-remodeling complexes can translocate nucleosomes along the DNA in an ATP-coupled reaction. This process is an important regulator of all DNA-dependent processes because it determines whether certain DNA sequences are found in regions between nucleosomes with increased accessibility for other factors or wrapped around the histone octamer complex. In a comparison of seven different chromatin-remodeling machines (ACF, ISWI, Snf2H, Chd1, Mi-2, Brg1, and NURF), it is demonstrated that these complexes can read out DNA sequence features to establish specific nucleosome-positioning patterns. For one of the remodelers, ACF, we identified a 40-bp DNA sequence element that directs nucleosome positioning. Furthermore, we show that nucleosome positioning by the remodelers ACF and Chd1 is determined by a reduced affinity to the end product of the translocation reaction. The results suggest that the linkage of differential remodeling activities with the intrinsic binding preferences of nucleosomes can result in establishing distinct chromatin structures that depend on the DNA sequence and define the DNA accessibility for other protein factors.
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