S-亚硝基化
半胱氨酸
拟南芥
生物
拟南芥
亚硝化
生物化学
一氧化氮
细胞生物学
功能(生物学)
半胱氨酸代谢
基因
突变体
酶
内分泌学
作者
Juliette Puyaubert,Abasse Fares,Nathalie Rezé,Jean-Benoı̂t Peltier,Emmanuel Baudouin
出处
期刊:Plant Science
[Elsevier]
日期:2014-02-01
卷期号:215-216: 150-156
被引量:120
标识
DOI:10.1016/j.plantsci.2013.10.014
摘要
S-nitrosylation is a nitric oxide (NO)-based post-translational modification regulating protein function and signalling. We used a combination between the biotin switch method and labelling with isotope-coded affinity tag to identify endogenously S-nitrosylated peptides in Arabidopsis thaliana proteins extracted from plantlets. The relative level of S-nitrosylation in the identified peptides was compared between unstressed and cold-stress seedlings. We thereby detected 62 endogenously nitrosylated peptides out of which 20 are over-nitrosylated following cold exposure. Taken together these data provide a new repertoire of endogenously S-nitrosylated proteins in Arabidopsis with cysteine S-nitrosylation site. Furthermore they highlight the quantitative modification of the S-nitrosylation status of specific cysteine following cold stress.
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