Large scale purification of linear plasmid DNA for efficient high throughput cloning

Abstract In this report we describe a rapid, simple, and efficient method for large‐scale purification of linear plasmid DNA to answer demand from high‐throughput gene cloning. The process is based on the separation of the linear vector from small DNA fragments by anion exchange chromatography. Gene cloning experiments by restriction/ligation or the In‐Fusion(tm) technique confirmed the high quality of the linearized vector as 100% of the genes were successfully cloned.