达尼奥
斑马鱼
孵化
结晶
X射线
生物
酶
动物
结晶学
化学
生物化学
物理
生态学
光学
基因
有机化学
作者
Ai Okada,Koji Nagata,Kôkichi Sano,Shigeki Yasumasu,Keiko Kubota,Jun Ohtsuka,Ichiro Iuchi,Masaru Tanokura
出处
期刊:Acta crystallographica
[International Union of Crystallography]
日期:2009-09-23
卷期号:65 (10): 1018-1020
被引量:6
标识
DOI:10.1107/s1744309109033016
摘要
The hatching enzyme of the zebrafish, ZHE1 (29.3 kDa), is a zinc metalloprotease that catalyzes digestion of the egg envelope (chorion). ZHE1 was heterologously expressed in Escherichia coli, purified and crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as the precipitant. Two diffraction data sets with resolution ranges 50.0-1.80 and 50.0-1.14 A were independently collected from two crystals and were merged to give a highly complete data set over the full resolution range 50.0-1.14 A. The space group was assigned as primitive orthorhombic P2(1)2(1)2(1), with unit-cell parameters a = 32.9, b = 62.5, c = 87.4 A. The crystal contained one ZHE1 molecule in the asymmetric unit.
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