过氧化物酶体
乙醛小体
生物
生物化学
拟南芥
亚细胞定位
基因
拟南芥
乙醛酸循环
过氧化物酶体靶向信号
酶
脂肪酸
微体
突变体
作者
Martin Fulda,Jay Shockey,Martin Werber,Frank P. Wolter,Ernst Heinz
出处
期刊:Plant Journal
[Wiley]
日期:2002-10-01
卷期号:32 (1): 93-103
被引量:159
标识
DOI:10.1046/j.1365-313x.2002.01405.x
摘要
Post-germinative growth of oilseeds is dependent on the breakdown of the stored lipid reserves. Long-chain acyl-CoA synthetase activities (LACS) are critically involved in this process by activating the released free fatty acids and thus feeding the beta-oxidation cycle in glyoxysomes. Here we report on the identification of two LACS genes, AtLACS6 and AtLACS7 from Arabidopsis thaliana coding for peroxisomal LACS proteins. The subcellular localization was verified by co-expression studies of spectral variants of the green fluorescent protein (GFP). While AtLACS6 is targeted by a type 2 (PTS2) peroxisomal targeting sequence, for AtLACS7 a functional PTS1 as well as a PTS2 could be demonstrated. Possible explanations for this potentially redundant targeting information will be discussed. Expression studies of both genes revealed a strong induction 1 day after germination resembling the expression pattern of other genes involved in beta-oxidation. Analysis of the substrate specificities of the two LACS proteins demonstrated enzymatic activity for both enzymes with the whole spectrum of fatty acids found in stored lipid reserves. These results suggest that both LACS proteins might have overlapping functions and are able to initiate beta-oxidation in plant peroxisomes.
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