痰
肺结核
色谱法
结核分枝杆菌
化学
固相萃取
气相色谱-质谱法
质谱法
气相色谱法
医学
病理
作者
Marta P.B. Mourão,Sjoukje Kuijper,Ngoc A. Dang,Elisabetta Walters,Hans‐Gerd Janssen,A. H. J. Kolk
标识
DOI:10.1016/j.jchromb.2015.12.023
摘要
Tuberculosis (TB) remains a worldwide health problem, especially in developing countries. Correct identification of Mycobacterium tuberculosis (MTB) infection is extremely important for providing appropriate treatment and care to patients. Here we describe a solid phase extraction-gas chromatography-mass spectrometry method (SPE-THM-GC-MS) for the detection of five biomarkers for M. tuberculosis. The method for classification is developed and validated through the analysis of 112 sputum samples from patients suspected of having TB. Twenty of twenty-five MTB culture-positive sputum samples were correctly classified as positive by our improved SPE-THM-GC-MS method. Eighty-five of eighty-seven MTB culture-negative samples were also negative by SPE-THM-GC-MS. The overall sensitivity of the new SPE-THM-GC-MS method is 80% (20/25) and the specificity is 98% (85/87) compared with culture. The method proved to be reliable and, although complex in principle, easy to operate due to the high degree of automation.
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