卵母细胞
能力(人力资源)
化学
细胞生物学
计算生物学
生物
心理学
胚胎
社会心理学
作者
Shefali Gupta,Sriti Pandey,Mehtab S. Parmar,Anjali Somal,Avishek Paul,B. Panda,Irfan Ahmad Bhat,Indu Baiju,Mukesh Kumar Bharti,G. Saikumar,Mihir Sarkar,Vikash Chandra,G. Taru Sharma
出处
期刊:Zygote
[Cambridge University Press]
日期:2017-06-01
卷期号:25 (3): 313-320
被引量:11
标识
DOI:10.1017/s0967199417000156
摘要
Oocyte-secreted factors (OSFs) play an important role in the acquisition of oocyte developmental competence through bidirectional cross-talk between oocyte and cumulus cells via gap junctions. Thus, the present study was designed to investigate the effect of two OSFs, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), on the developmental competence of buffalo oocytes derived from two different follicle sizes. Cumulus-oocyte complexes (COCs) from large follicles (LF, >6 mm) or small follicles (SF, 0.05) between DOs and combination groups. Relative mRNA analysis revealed significantly higher (P > 0.05) expression of the cumulus cell marker genes EGFR, HAS2, and CD44 in LF-derived than SF-derived oocyte; the expression of these markers was significantly higher (P > 0.05) in DOs and combination groups, irrespective of the follicle size. These results suggested that LF-derived oocytes have a higher developmental competence than SF-derived oocytes and that supplementation of GDF9 and BMP15 modulates the developmental competence of buffalo oocytes by increasing the relative abundance of cumulus-enabling factors and thereby increasing cleavage and the quality of blastocyst production.
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