Purpose The primary objective of this study is to record and document the current rate and reason for donor deferral in our tertiary care hospital in Southern Rajasthan. Method A retrospective study of donors was carried out from the donor records in the blood bank of a tertiary hospital during period of April 2014 to April 2016. Detailed information of the donor deferral was recorded from deferral register. We used statistical method to detect the rate and reason for donor deferral. Result and Conclusion Every blood bank should analyze the reasons of deferral amongst blood donors and utilize this analysis for addressing the issue and ameliorating the cause of deferral if possible.