Progress in the preparation of siRNAs

Recently, siRNAs (small interfering RNAs) have been widely used to induce RNA interference inmammalian systems. Till now, there are five methods to produce siRNAs, such as chemical synthesis, in vitrotranscriptions, RNase III-family enzymatic digestion, siRNA expression vectors and siRNA expression cassettes.It is essential to choose an effective siRNA sequence in those methods. Furthermore, with the developments ofdrug discovery and genomics, the siRNAs preparation should be developed and consummated in severalways, such as high-throughput screening of effective siRNAs, stability of siRNAs, efficiently gene transferand regulation of the expression of siRNAs.