Comparison of High-fat Diet and Methionine-choline Deficient diet induced by non-alcoholic Steatohepatitis Animal Model

Objective: Comparison of high-fat diet and methionine-choline deficient diet induced by non-alcoholic steatohepatitis animal model in serology and pathology, and explore the pathogenesis and mechanism. Methods: SD rats feed high-fat diet and C57BL/6 mice feed methionine-choline deficient diet to become model of non-alcoholic steatohepatitis. Liver tissue and blood were harvested after 8 weeks(rats) and 2 weeks(mice), which were detected by TG, CHO, FPG, FINS, HE and oil-red O. At last, the rats were assessed by NAFLD Activity Score(NAS) and HOMA-IR. Results: The levels of serum TG, CHO, FPG, FINS, HOMA-IR were significantly increased in NASH rats. The levels of serum TG, CHO were significantly decreased in NASH mice. The results of HE, oil-red O and NAS show liver tissue of rats and mice have been developed to NASH. Conclusion: Two methods can be simulated human NASH disease in serology and pathology, high-fat diet induced by NASH model can simulate the process and mechanism of human NASH disease, and this model can copy insulin resistance and oxidative stress, so this method have more advantage in NASH research field.