Effect of by overexpressing gumD in Xanthomonas campestris on the xanthan gum

Purpose To improve the yield and quality of xanthan gum by overexpressing gumD in Xanthomonas campestris 58(Xc58).MethodsBy PCR amplification,plasmid construction,triparental conjugation and other methods,pBBR-gumD was transformed into the original strain Xc58.ResultsCompared with Xc58,the recombinant strain Xc58-D has increased by 11.19% in the yield of xanthan gum,by 6.31% increased in viscosity,by 20.21 % increased in molecular weight,and by 77.07% increased in acetyl content,but 6.34% decreased in pyruvate content.ConclusionThe recombinant strain has a higher yield and improves the quality of xanthan gum.