The extraction technology of total polyphenol in Amaranthus spinosus was optimized and the effect of scavenging hydroxyl radicals was studied to lay the foundation of theoretical basis for research and development of Amaranthus spinosus.The results showed that the optimum extraction technological condition was 60% ethanol,1∶10 material-liquid ratio,40℃ extraction temperatre and 1h extraction time.The extraction rate of total polyphenol from Amaranthus spinosus can reach 4.89mg/g under the optimum extraction technology.The rate of scavenging hydroxyl radicals can be up to 60% when total polyphenol concentration is 2.5μg/mL