Protective effects of penehyclidine hydrochloride against acute renal injury induced by hemorrhagic shock and lipopolysaccharides in rats

Objective To investigate the effect of penehyclidine hydrochloride(PHC) in a rat model of renal injury induced by hemorrhagic shock and lipopolysaccharides(LPS) . Methods Forty-five healthy Wistar rats were randomized into sham operated group,model group,and 3 penehyclidine hydrochloride(PHC) dose(1,2 and 3 mg/kg) groups(PHC1,PHC2,and PHC3 groups,respecitvely) . The arterial blood samples were collected to determine the concentrations of serum tumor necrosis factor-α(TNF-α) ,interleukin-8(IL-8) ,interleukin-1(IL-1) ,urine creatinine(Cr) and blood urine nitrogen(BUN) ,and the renal tissues were collected to measure the expressions of ICAM-1 and nuclear factor-κB(NF-κB) and observe the pathological changes. Results TNF-α,IL-8,IL-1,Cr,BUN,ICAM-1 and NF-κB in the 3 PHC groups were significantly lower than those in the model group(P0.05) . TNF-α,IL-8,IL-1,Cr and BUN were significantly lower in PHC1(P0.05) than in the PHC2 and PHC3 groups,and ICAM-1 and NF-κB were similar between 3 PHC groups(P0.05) . Compared with the model group,the 3 PHC groups showed lessened pathological changes in the renal tubules. Conclusion PHC has protective effects against renal injury induced by hemorrhagic-endotoxin shock in rats,and treatment with 1 mg/kg PHC produces the most significant protective effect.