噬菌体展示
肽库
肽
胶体金
流式细胞术
细胞毒性
癌细胞
化学
癌症研究
分子生物学
细胞生物学
生物
体外
生物化学
癌症
纳米技术
肽序列
材料科学
纳米颗粒
基因
遗传学
作者
Elisabetta Galbiati,Luca Gambini,Viola Civitarese,Melissa Bellini,Dario Ambrosini,Raffaele Allevi,Svetlana Avvakumova,Sergio Romeo,Davide Prosperi
标识
DOI:10.1016/j.phrs.2016.06.007
摘要
Tumor homing peptides (THPs) specific for a representative breast cancer cell line (MCF-7) were carefully selected basing on a phage-displayed peptide library freely available on the web, namely the TumorHoPe: A Database of Tumor Homing Peptides. The selected THPs were synthesized and evaluated in terms of their affinity toward MCF-7 cells. Out of 5 tested THPs, 3 best-performing peptide sequences and 1 scrambled sequence were separately conjugated to spherical gold nanoparticles yielding stable nanoconjugates. THP nanoconjugates were examined for their ability to actively target MCF-7 cells in comparison to noncancerous 3T3-L1 fibroblast cells. These THP-gold nanoconjugates exhibited good selectivity and binding affinity by flow cytometry, and low cytotoxicity as assayed by cell death experiments. The uptake of targeted nanoconjugates by the breast cancer cells was confirmed by transmission electron microscopy analysis. This work demonstrates that it is possible to exploit the conjugation of short peptides selected from phage-displayed libraries to develop nanomaterials reliably endowed with tumor targeting potential irrespective of a specific knowledge of the target cell biology.
科研通智能强力驱动
Strongly Powered by AbleSci AI