The application research of HFC-365mfc foaming agent in rigid polyurethane foam was done in this article.The results showed that mechanical properties of foam were quite close between HFC-365mfc and HCFC-141b.The results also indicated mechanical properties of rigid polyurethane foam using HFC-365mfc could reach standard of the spray polyurethane foam for thermal insulation (JC/T998-2006) and foaming agent HFC-365mfc could replace HCFC-141b in application.