Molecular Detection of Y Chromosome for the Patients with Idiopathic Azoospermia and Severe Oligozoospermia

Objective:To study the relationship between microdeletion on Y chromosome and the patients with idiopathic azoospermia and severe oligozoospermia and establish the molecular detection method for the patients with azoospermia and severe oligozoospermia. Methods: Microdeletion detection at the AZFa?AZFb?AZFc/DAZ?SRY region of Y chromosome in 72 azoospermia and 28 severe oligozoospermia patients was performed using the PCR technique. Results: Twelve patients with AZFc/DAZ micodeletion, including 8 azoospermia(11.1% )and 4 severe oligozoospermia(14.3%), had been found and 1 patient with AZFb and AZFc/DAZ double deletion had been found; the deletion of AZFa and SRY region hadn't been found. The deletion of AZFa?AZFb?AZFc/DAZ?SRY region hadn't been found in 60 normal men with children. Conclusion: Microdeletion on Y chromosome, especially AZFc/DAZ, is a major cause of azoospermia and severe oligozoospermia leading to male infertility. It is necessary that we detect microdeletion on Y chromosome when genetic counseling and ICSI.