Next generation sequencing technology and its application in cancer research

The next generation sequencing(NGS),also known as high-throughput sequencing technology,is a landmark of revolution in the history for DNA sequencing.NGS have adopted a new sequencing strategy—cyclic-array sequencing,which was divided into pyrosequencing(Roche/454),Solexa sequencing technology(Illumina),the polony sequencing technology(ABI/ SOLiD),Polonator sequencing technology and HeliScope single-molecule sequencing technology.Because of its high-throughput,low cost and short time-consuming,NGS has the potential to replace traditional Sanger sequencing and to become the most important research tools in post-genomic era.Researchers could do more comprehensive and in-depth analysis with genome,transcriptome and proteome at cheaper price.NGS has broad application prospects in the field of cancer research.In addition to accomplishing the tumor genome sequencing quickly,it also provides us a fully and rapidly powerful digital tool for tumor transcriptome,epigenetic and DNA-protein interaction analysis.It is expecting to bring about revolutionary change in the areas of life science,medicine science and others.