化学
邻苯三酚
没食子酸
大肠杆菌
色谱法
生物化学
生物合成
凝胶电泳
高效液相色谱法
核化学
酶
抗氧化剂
基因
作者
Dingkun Zhang,Toshiyoshi Yamamoto,Dewei Tang,Yuriko Kato,Shiho Horiuchi,Shinya Ogawa,Esturo Yoshimura,Michio Suzuki
出处
期刊:Talanta
[Elsevier]
日期:2019-04-01
卷期号:195: 447-455
被引量:16
标识
DOI:10.1016/j.talanta.2018.11.092
摘要
In this work, CdS nanoparticles (CdS NPs) biosynthesized through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli (CdS/AtPCS1-E. coli) with fluorescence (FL) performance for detection of pyrogallol and gallic acid was investigated. Through expression of the AtPCS1 gene inside E. coli cells by pET28b vector, biosynthesis of CdS NPs was greatly enhanced due to generation of phytochelatins (PCs, (γ-Glu-Cys)n-Gly, n ≥ 2) for efficient capture of Cd2+. The expression of AtPCS1 and concentration of glutathione (GSH) and PCs were detected by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high performance liquid chromatography (HPLC), respectively. The morphology and component were checked through scanning electron microscope (SEM), transmission electron microscopy (TEM) and energy dispersive spectrometer (EDS). FL effect with different experimental conditions were examined. In addition, it is also applied to determination of pyrogallol and gallic acid. These results revealed that multifunctional PCs could effectively facilitate biosynthesis of CdS NPs with higher yield, better distribution and lower cost while stronger FL intensity could be obtained for quantitative analysis.
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